PCR Protocol--Regenerate PCR

Degenerate PCR

Degenerate PCR
The identification of novel members of gene families by PCR using degenerate primers is described and protocols given. Article by Michael Koelle 1996 on the ...www.dartmouth.edu/~ambros/protocols/other/koelle/degenerate_PCR.html
Degenerate PCR.html (Michael Koelle)
by Michael Koelle. The identification of novel members of gene families by PCR using degenerate primers has been considered more of an art than a science, ...www.med.yale.edu/mbb/koelle/protocols/protocol_degenerate_PCR.html
PCR Primer Design
However, I have used primers with as high as 256- and 1024-fold degeneracy for the successful amplification and subsequent direct sequencing of a wide range ...www.mcb.uct.ac.za/pcroptim.htm
Degenerate PCR (NTNU Cell and Molecular Biology Group)
X - Services · Open Positions, Degenerate PCR Degenerate PCR is in most respects identical to ordinary PCR, but with one major difference. ...http://boneslab.bio.ntnu.no/degpcrshortguide.htm
Designing degenerate PCR primers
The purpose of CODEHOP is to help in the design of degenerate PCR primers from protein sequence. Given a multiple alignment of a series of target proteins, ...genomebiology.com/2000/1/1/reports/240
Nature Protocols: Quantitative multiplex degenerate PCR for human endogenous retrovirus expression profiling.
Last, the protocol below provides general rules for the design of MD-PCR applications. Once primers have been designed and optimized, the procedure can be ...www.natureprotocols.com/2007/01/25/quantitative_multiplex_degener.php