BioProtocols for Nucleic Acids (DNA-1)

DNA (In Alphabeta)

ABC Random Amplification
Alcohol Precipitation of DNA
AML sample DNA preparation

Agarose gel electrophoresis

Agarose gel electrophoresis

Boomerang DNA Amplification Single primer DNA amplification

cDNA amplification from l-phage library

cDNA library screening

Characterization of DNA

Collection of Materials for DNA Analysis

Colony Hybridization

Colony Hybridization Protocols

Deoxyribose Isolation from DNA Degrasion

DNA Isolation, Purification and Troubleshooting

DNA Labeling Protocols

DNA ligation Protocol

DNA Methods （3）

DIG DNA labelling and hybrid detection (Boehringer Mannheim)

Direct Automated Sequencing off Bacterial Genomic DNABruce Rhoe

DNA extraction from agarose (Qiagen)

DNA - dische diphenylamine determination

DNA Fragment Isolation from LM agarose

DNA Ligation

DNA detection
DAPI chromosome identification

DNA preparation by cryostom tissue dissection
DNA labeling by nick translation

Ethanol precipitation of DNA

Extraction of DNA from bovine spleen

Fluorometry

BioProtocols for Nucleic Acids (DNA-2)

FISH

Genome DNA Extraction

Genomic DNA Isolation from Specific Samples

Genomic DNA preparation (plants)
Hybridization

In-Situ hybridization to Embryonic Tissue Section

Large Scale Plasmid Preparation: MGH

Methylene Blue DNA staining protocol

Microsatellites Protocols

Mitochondrial DNA Isolation

Modifications of DNA ends

PCR amplification of DNA

PCR analysis of DNA from Laser Microdissected (LM) Samples - Embryo Genotyping Protocol

PCR product purification (Qiagen)

Perfectly Blunt Cloning of DNA (Novagen)

Phenol/chloroform extraction of DNA

Plasmid midi-prep from bacteria

Plasmid Protocol--(1-10)

Plasmid Protocols--(21-30)

Plasmid Protocols--(31-37)

Preparation of genomic DNA from bacteria

Purification of DNA

Q-PCR

Random Primed labeling of DNA (Prime-It II -Stratagene)

Restriction enzyme digestion of DNA
Southern Hybridization
Stark's Prehybridization Buffer

Sequencing using Sequenase

Southern blot

Tail DNA prep

Wizard Plus Mini-preps (109. Plasmid Mini-prep)

Worm Genomic DNA: MGH

Worm genomic Southern blots: Michael Koelle

BioProtocols for Nucleic Acids (RNA)

RNA (In Alphabeta)

Amino-allyl labeling
BioAnalyzer

C. elegans RNA prep: Michael Koelle

IN VITRO TRANSCRIPTION OF RNA

In vitro transcription reaction

Isolation of poly-A RNA

LiCl RNA Preparation: MGH

Northern blots

Northern Blots: Michael Koelle

Nucleic Acids Research Methods (2)

Nucleotide composition of RNA

Orcinol determination of RNA

Phenol extraction of rRNA (rat liver)

PROTOCOL FOR SINGLE CELL mRNA AMPLIFICATION (REVERSE NORTHERN ANALYSIS)

Protocol: Fluorescence in situ hybridization

Protocols for SiRNA

Reverse Transfection Protocols

RNA amplification
RNA gel electrophoresis

RNA and protein extraction from the same sample

RNA Isolation

RNA MicroArray Protocol

RNA prepararion (from plants tissue)

RNA Preparation: MGH

RNEasy Midi

SELEX—in vitro selection

shRNA Protocols

Spectrophotometric analysis of RNA

Sucrose density fractionation

TRIzol plus RNEasy Mini
TRIzol product insert

Worm RNA Preparation: MGH

Nucleic Acids Research Articles and Protocols

Cell biology (8 Articles)

Chromatin (4 Articles)

Cloning (35 Articles)

Computational methods (92 Articles)

DNA characterisation (96 Articles)

DNA transfer (30 Articles)

Enzyme assays (24 Articles)

Genomics (74 Articles)

Microarray (48 Articles)

Miscellaneous/other (62 Articles)

Monitoring gene expression (63 Articles)

Mutagenesis (42 Articles)

New Restriction Enzymes (17 Articles)

Nucleic acid amplification (66 Articles)

Nucleic acid modification (42 Articles)

Nucleic acid structure (20 Articles)

Polymorphism/mutation detection (112 Articles)

Protein-nucleic acid interaction (50 Articles)

Protein-protein interaction (16 Articles)

Recombinant DNA expression (24 Articles)

Recombination (54 Articles)

Repair (20 Articles)

Replication (13 Articles)

RNA characterisation and manipulation (59 Articles)

Targeted inhibition of gene function (14 Articles)