BioProtocols for Nucleic Acids (DNA-2)

FISH

Genome DNA Extraction

Genomic DNA Isolation from Specific Samples

Genomic DNA preparation (plants)
Hybridization

In-Situ hybridization to Embryonic Tissue Section

Large Scale Plasmid Preparation: MGH

Methylene Blue DNA staining protocol

Microsatellites Protocols

Mitochondrial DNA Isolation

Modifications of DNA ends

PCR amplification of DNA

PCR analysis of DNA from Laser Microdissected (LM) Samples - Embryo Genotyping Protocol

PCR product purification (Qiagen)

Perfectly Blunt Cloning of DNA (Novagen)

Phenol/chloroform extraction of DNA

Plasmid midi-prep from bacteria

Plasmid Protocol--(1-10)

Plasmid Protocols--(21-30)

Plasmid Protocols--(31-37)

Preparation of genomic DNA from bacteria

Purification of DNA

Q-PCR

Random Primed labeling of DNA (Prime-It II -Stratagene)

Restriction enzyme digestion of DNA
Southern Hybridization
Stark's Prehybridization Buffer

Sequencing using Sequenase

Southern blot

Tail DNA prep

Wizard Plus Mini-preps (109. Plasmid Mini-prep)

Worm Genomic DNA: MGH

Worm genomic Southern blots: Michael Koelle