Molecular Biology Laboratory Manual

Beginning Molecular Biology Laboratory Manual

CHAPTER 1: General Laboratory Methods

CHAPTER 2: Instructions for Notebook Keeping

CHAPTER 3: Vector NTI User's Guide

CHAPTER 4: Molecular Biology Methods

Preparation of genomic DNA from bacteria

PCR amplification of DNA

Restriction enzyme digestion of DNA

Phenol/chloroform extraction of DNA

Ethanol precipitation of DNA

Agarose gel electrophoresis

Transformation of E. coli by electroporation

Preparative DNA Fragment Isolation from an Agarose Gel

Ligations of plasmid DNA to insert DNA

Transfection of mammalian cells using Lipofectamine

Southern blotting

Western Blot analysis of His-tagged proteinsl

Cycle Sequencing Protocols For ABI 3100

Colony PCR

One Step Gene Assembly (Gene Synthesis)

CHAPTER 5: Tissue Culture Methods

Types of cells grown in culture

Work area and equipment

Preservation and storage

Maintenance

Safety considerations

Tissue culture procedures

Determining cell counts

Common Stock Solutions