PCR Protocol--RAPD PCR

RAPD PCR

RAPD PCR
RAPD stands for Random Amplification of Polymorphic DNA, where the target sequence(s) (to be amplified) is unknown.Brief description, from Rutgers ...avery.rutgers.edu/WSSP/StudentScholars/project/archives/onions/rapd.html
RAPD PCR Reaction Mixes:
Type, Initialize, X, Denature, Anneal, Extend, Termination. RAPD 10-mer, 95C, 5min, 45, 94C, 0:30, 35C, 1:00, 72C, 2:00, 72C, 5:00. PCR 18+mer, 95C, 5:00 ...wheat.pw.usda.gov/~lazo/methods/lazo/pcrmeth0.html
Handbook for DNA isolation, RAPD-PCR and PCR-RFLP
A standard RAPD-PCR reaction is performed in volumes of 25.0 m l containing 50 mM KCl, 10 mM Tris-HCl pH 9.0, 1.5 mM MgCl2, 0.001% gelatine, ...www.toyen.uio.no/botanisk/brochmann/handbook.htm
RAPD Protocol dNTP mix (5mM): Loading buffer (6X): Ladder mix: PCR .RAPD Protocol. Protocol for Promega. and Stoffel brand Taq. enzymes (1 reaction):. 4 µl dNTP (5mM) mix. 2.5 µl 10X PCR buffer. 8.33 µl H ...www.css.msu.edu/bean/PDF/RAPD_protocol.pdf
PCR PROTOCOL FOR RAPDs--1PCR PROTOCOL FOR RAPDs. Application: Fingerprinting, molecular mapping, ... Amount of DNA needed per RAPD reaction according to the plant species under ...gene4.ciat.cgiar.org/limsys3.0/Modelos/protocolos/PCRPROTOCOLFORRAPDs.doc

PCR Protocol--RACE PCR

RACE PCR

Background: Rapid amplification of cDNA ends (RACE) is a polymerase chain reaction-
based technique which facilitates the cloning of full-length cDNA
sequences when only a partial cDNA sequence is available. Traditionally,
cDNA sequence is obtained from clones isolated from plasmid or
phage libraries. Frequently these clones lack sequences corresponding to
the 5' ends of the mRNA transcripts. The missing sequence information
is typically sought by repeatedly screening the cDNA library in an effort
to obtain clones that extended further towards the 5' end of the message.
The nature of the enzymatic reactions employed to produce cDNA
libraries limits the probability of retrieving extreme 5' sequence even
from libraries that are very high quality.

RACE-PCR facilitates the isolation of 5 and 3 end sequences from cDNA.
Human Molecular Genetics 2 conveys the feel of fast-moving research while providing a description in some depth of the techniques and data that are helping ...www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=hmg.figgrp.2574
5' RACE PCR
5' RACE PCR Protocol 1. First strand cDNA synthesis: - Reaction set up: cDNA synthesis buffer 4 ul dNTP mixture 2 ul gene specific primer 1 1 ul ...biowww.net/detail-126.html
3' RACE PCR
Detailed protocol on 3' RACE PCR - rapid amplification of 3' cDNA end. ... 3' RACE PCR Protocol: 1. First strand cDNA synthesis: ...biowww.net/detail-329.html SMART RACE cDNA Amplification Protocol-at-a-GlanceProtocol-at-a-Glance. 2. For 5'-RACE and 3'-RACE: prepare PCR reactions as shown in Tables III & IV. Add the components in the order shown in PCR tubes. ...www.clontech.com/images/pt/PT3269-2.pdf
5' RACE Protocol For Generation of Seq. Tags.Apply all 79 ul of1st PCR product to center of packed wells. ... Sequence template per 5'RACE sequencing protocol with sequencing oligo 305. ...baygenomics.ucsf.edu/protocols/comp1/RACE_generation.pdf
Rapid Amplification of cDNA 3' Ends (3'- RACE)( Simplified protocol based on Clontech’s SMART- RACE ). 6. Distribute 5 µl of diluted first PCR product in the PCR tubes, 8. strips or 96 plate on ice. ...www.cmhd.ca/protocols/genetrap_pdf/3'RACE.pdf
A lock-docking oligo(dT) primer for 5' and 3' RACE PCR.
stretch of As, yielding a discrete PCR. product. We are planning to use the lock-dock-. ing primer in the 5'-RACE protocol (1) to ...www.genome.org/cgi/reprint/2/2/144.pdf
First Choice RACE-Ready cDNA ProtocolThe 3' RACE protocol. describes nested PCR, however 3' RACE reactions may produce signif- ..... your PCR protocol. Figure 3. 3' RACE Control. 3' Adapter ...http://www.ambion.com/techlib/prot/fm_3200.pdf
Nature Protocols: 3′ End cDNA amplification using classic RACE
... amplification of cDNA ends (RACE) PCR as long as part of the mRNA sequence is known; ... cDNA ends can be obtained in 1–3 days using this protocol. ...www.natureprotocols.com/2007/01/11/3_end_cdna_amplification_using.php
High throughput Rapid Amplification of cDNA Ends (RACE) using cDNA Library as Template.Follow standard protocol for plasmid prep in microtitterplates. PCR for RACE-clones:. Use standard protocol for PCR, but use 1 ul plasmidprep as template ...www.bio.aau.dk/download/biotechnology/kln/RACE-procedures.doc
Help for 3'-RACE PCR
Help for 3'-RACE PCR. PROBLEM: We have prolem to amplify the 3'-end of our .... I intend to try the following: a) Modified an inverse PCR protocol by ...
http://wheat.pw.usda.gov/~lazo/methods/lazo/met11.html

PCR Protocol--Multiplex PCR

Multiplex PCR

PCR and multiplex PCR guideOctavian Henegariu,Yale University
Multiplex PCR
A step by step protocol
Troubleshooting for PCR and Multiplex PCR
Octavian Henegariu,Yale University
Multiplex PCR Handbook
Qiagen
Multiplex PCR System Protocol
GenScript
MultiPlex PCR-based Sequencing
Bruce Roe
Efficient Multiplex PCR Optimization - Online
Bioscience Tech
Multiplex PCR-based reverse line blot hybridization assay.
Nature protocols
Multiplex PCR with TaqMan VIC Probes
http://www3.appliedbiosystems.com/cms/groups/mcb_support/documents/generaldocuments/cms_041019.pdf
AB Applied Biosystems
Multiplex PCR (Chamberlain and Beggs)
Leiden Muscular Dystrophy pages

PCR Protocol--Regenerate PCR

Degenerate PCR

Degenerate PCR
The identification of novel members of gene families by PCR using degenerate primers is described and protocols given. Article by Michael Koelle 1996 on the ...www.dartmouth.edu/~ambros/protocols/other/koelle/degenerate_PCR.html
Degenerate PCR.html (Michael Koelle)
by Michael Koelle. The identification of novel members of gene families by PCR using degenerate primers has been considered more of an art than a science, ...www.med.yale.edu/mbb/koelle/protocols/protocol_degenerate_PCR.html
PCR Primer Design
However, I have used primers with as high as 256- and 1024-fold degeneracy for the successful amplification and subsequent direct sequencing of a wide range ...www.mcb.uct.ac.za/pcroptim.htm
Degenerate PCR (NTNU Cell and Molecular Biology Group)
X - Services · Open Positions, Degenerate PCR Degenerate PCR is in most respects identical to ordinary PCR, but with one major difference. ...http://boneslab.bio.ntnu.no/degpcrshortguide.htm
Designing degenerate PCR primers
The purpose of CODEHOP is to help in the design of degenerate PCR primers from protein sequence. Given a multiple alignment of a series of target proteins, ...genomebiology.com/2000/1/1/reports/240
Nature Protocols: Quantitative multiplex degenerate PCR for human endogenous retrovirus expression profiling.
Last, the protocol below provides general rules for the design of MD-PCR applications. Once primers have been designed and optimized, the procedure can be ...www.natureprotocols.com/2007/01/25/quantitative_multiplex_degener.php

PCR Protocol--Inverse PCR

Inverse PCR

Inverse PCR & Cycle Sequencing of P Element Insertions for STS GenerationJay Rehm
Inverse PCR for PAC-end sequencingBrad Barbazuk
Inverse PCR:For use with Snyder mTn-lacZ/LEU2 based mutagenesisFred Hutchinson Cancer Research Center
Cloning flanking DNA from pD991 enhancer trap T-DNA insertsDepartment of Biological Sciences Dartmouth College

Inverse PCR protocol
Maddock Lab.

Inverse PCR and Sequencing Protocol
E. Jay Rehm

Inverse PCR
Trevor Epp

Springer Protocols: Inverse PCR (IPCR) for Obtaining Promoter Sequence.
Springer Protocols

PCR Protocol--PCR Elisa

What is PCR Elisa?
PCR products are labeled (e.g., by digoxigenin) during amplification. A capture probe specific to the PCR amplicon is used to immobilize the amplicon to well plate. ELISA against the label (e.g., anti-digoxigenin) is then used to quantitate PCR products.

PCR-ELISA, an alternative to real time PCR for detecting.
PCR-ELISA is an alternative to real time PCR used to detect sequences within the polymerase chain reaction products.www.btc-bti.com/pcrelisa.htm

Mycoplasma Detection by the Mycoplasma PCR ELISAMycoplasma Detection by the. Mycoplasma PCR ELISA. Introduction. The most frequent contaminants of animal ...www.roche-applied-science.com/PROD_INF/BIOCHEMI/jlp33-35.pdf B

Telomerase PCR ELISATelomerase PCR ELISA. For fast and sensitive detection of ... The indicated number of cells were analyzed in the Telomerase PCR ELISA.Assays were ...www.roche-applied-science.com/PROD_INF/BIOCHEMI/No.4_96/p7-8.pdf

Optimisation of one-tube PCR Elisa to detect femtogram amounts of genomic DNA.
A simple, high-throughput, low-cost polymerase chain reaction-enzyme linked immunosorbent assay (PCR-ELISA) protocol that detects the presence of 4 fg of ...linkinghub.elsevier.com/retrieve/pii/S0167701202000556

Qualitative PCR-ELISA protocol for the detection and typing of viral genomes.
Nature Protocols is an interactive online resource for all laboratory protocols relevant to biological and biomedical research. Nature Protocols includes a ...npg.nature.com/nprot/journal/v2/n10/abs/nprot.2007.311.html

PCR Protocol--Nested PCR

Nested PCR

What is nested PCR? Nested PCR is a variation of the polymerase chain reaction (PCR), in that two pairs (instead of one pair) of PCR primers are used to amplify a fragment.

The first pair of PCR primers amplify a fragment similar to a standard PCR. However, a second pair of primers called nested primers (as they lie / are nested within the first fragment) bind inside the first PCR product fragment to allow amplification of a second PCR product which is shorter than the first one.
The advantage of nested PCR is that if the wrong PCR fragment was amplified, the probability is quite low that the region would be amplified a second time by the second set of primers. Thus, Nested PCR is a very specific PCR amplification.

PCR amplification of cDNA segments by 2 stage nested PCRprotocolNCBI
Standard Nested PCR ProtocolC. elegans Gene Knockout Project, Vancouver Lab
Poison Primer Nested PCR Protocol
Moerman Lab.
Nested PCR and Sequencing of T-DNA Junctions in Arabidopsis
http://signal.salk.edu/T-DNArecovery.pdf
ECKER LAB
Protocol for nested PCR amplification.